Single Molecule FLIM at Minimal Invasive Conditions

5 W/cm2 average power, observed with multi-anode detector and TIRF

QA-movies: single DsRed-1 molecules: adsorbed (in focus, left) and freely diffusing (out of focus). Adsorbed molecules display site-dependent, 2-exponential fluorescence dynamics.

QA-movie of single molecule tracking: both specimens display different lifetime:
2 ns and 3 ns